Recombinant human TIM3 protein aa1-201 was produced in mammalian cells with C-terminal His-DYK tag. The theoretical molecular weight of recombinant TIM3 is 22.1 kDa. Purified TIM3 protein is between the position of 43-26 kDa on SDS-PAGE gel under reduced condition.
TIM3 protein is purified by proprietary chromatographic techniques.
Entrez Gene summary for TIM3:
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
UniProtKB/Swiss-Prot: Q8TDQ0 (HAVR2_HUMAN)
Function: Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function.
别名 T-cell immunoglobulin and mucin domain-containing protein 3 / TIMD-3
T-cell immunoglobulin mucin receptor 3 / TIM-3
T-cell membrane protein 3
形态 Protein is supplied in 20 mM Tris,150 mM NaCl, 10% Glycerol, pH 7.5。
Accession # NP_116171.3
保存方法 Clear solution, frozen.Store at -70°C. At first use, aliquot and store at -70°C to avoid multiple freeze-thaws.
序列 TIM3-His-DYK (N-terminal 21 aa is predicted to be signal peptide): MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRIHHHHHHHHDYKDDDDK