GAPDH Polyclonal Antibody
|反应种属 ||Human Mouse Rat|
|应用||WB IHC IF|
|抗原来源||Recombinant protein of human GAPDH|
|背景||Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transport, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PACR and also loading control in electrophoresis and Western blotting. This antibody is a rabbit polyclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.|
|保存方法||Store at -20℃. Avoid freeze / thaw cycles.|
|存储溶液||Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Immunohistochemistry of paraffin-embeddedrat pancreas using GAPDH antibody at dilution 1:100(400x lens).