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Recombinant Rat IFN-γ

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  • 产品型号:abs01082
  • 厂商性质:生产厂家
  • 更新时间:2024-03-04
  • 访  问  量:865

详细介绍

品牌absin规格20ug
供货周期一周
概述
种属Rat
形态Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80.
来源Escherichia coli.
氨基酸序列QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC
内毒素水平Less than 0.1 EU/µg of rRtIFN-γ as determined by LAL method.
活性Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Accession #P01581
预期分子量Approximay 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
性能
溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度> 97 % by SDS-PAGE and HPLC analyses.
Gene IDs25712
参考文献
1. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>2. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.<br/>3. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>4. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.

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