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首页 > 产品中心 > 常规生化试剂 > 趋化因子及其受体 > abs04172PBEF/Visfatin (N-6His)

PBEF/Visfatin (N-6His)

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二抗、标签抗体、对照抗体;WB相关:ECL发光液、预染marker、预制胶;IHC相关:二抗试剂盒、组化笔;IP/CoIP试剂盒;细胞因子、信号通路调节剂;血清、BSA、蛋白酶K、CTB、TTX、CEE;凋亡试剂盒、呼吸爆发试剂盒;动植物提取物...

  • 产品型号:abs04172
  • 厂商性质:生产厂家
  • 更新时间:2022-11-15
  • 访  问  量:560

详细介绍

品牌自营品牌规格10ug
供货周期一周
概述
描述Recombinant Human Visfatin is produced by our E.coli expression system and the target gene encoding Met1-His491 is expressed with a 6His tag at the N-terminus.
别名Pre-B cell-enhancing factor, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, NAMPT, PBEF, PBEF1
形态Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.
来源E.coli
氨基酸序列MNHKVHHHHHHMNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKY EETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAV PEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLD GLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHST ITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDS GNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIA FGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLE EGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
内毒素水平Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Accession #P43490
性能
背景Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “visfatin”. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
纯度Greater than 95% as determined by reducing SDS-PAGE. 

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