详细介绍
| 品牌 | absin | 纯度 | > 95 % by SDS-PAGE and HPLC analyses. |
|---|---|---|---|
| 货号 | abs00996 | 规格 | 10ug |
| 供货周期 | 现货 | 主要用途 | Lyophilized from a 0.2 μm filtered solu |
| 应用领域 | 化工,生物产业,农林牧渔,制药/生物制药,综合 |
Recombinant Mouse M-CSF Protein
| 概述 | |
| 别名 | M-CSF蛋白;CSF1蛋白 |
| 种属 | Murine |
| 来源 | Escherichia coli. |
| 形态 | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
| 性能 | |
| 序列 | KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
| 标签 | No Tag |
| 纯度 | > 95 % by SDS-PAGE and HPLC analyses. |
| 内毒素水平 | Less than 1 EU/μg of rMuM-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 储存/保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 靶标 | |
| Accession | Q3U4F9 |
| 参考文献 | |
| 参考文献 | 1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26. 2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52. 3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35. 4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102. |
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