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Recombinant Human VEGF165(10ug)

Recombinant Human VEGF165(10ug)

型    号: abs04187
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主要产品线:生化试剂(60万种)、抗体(10万种)、细胞因子、二抗、ECL发光液、蛋白marker、激动剂、抑制剂、凋亡试剂盒、BSA、动植物提取剂、蛋白酶K....

abs04187Recombinant Human VEGF165(10ug)的详细资料:

概述
描述Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
别名Vascular Endothelial Growth Factor Isoform 165, VEGF165
形态Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
来源Human Cells
氨基酸序列APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEG LECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQ DPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
内毒素水平Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Accession #P15692-4
性能
背景Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the plaet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
纯度Greater than 95% as determined by reducing SDS-PAGE.

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