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详细介绍
| 品牌 | absin | 纯度 | > 95 % by SDS-PAGE and HPLC analyses. |
|---|---|---|---|
| 货号 | abs00875 | 规格 | 10ug |
| 供货周期 | 现货 | 主要用途 | Lyophilized from a 0.2 μm filtered conce |
| 应用领域 | 化工,生物产业,农林牧渔,制药/生物制药,综合 |
Recombinant Human aFGF Protein
| 概述 | |
| 别名 | aFGF蛋白 |
| 种属 | Human |
| 来源 | Escherichia coli. |
| 形态 | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| 性能 | |
| 序列 | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
| 标签 | No Tag |
| 纯度 | > 95 % by SDS-PAGE and HPLC analyses. |
| 内毒素水平 | Less than 1 EU/μg of Recombinant HumanaFGF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/ml of heparin. |
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 储存/保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 靶标 | |
| Accession | P05230 |
| Gene IDs | 2246 |
| 参考文献 | |
| 参考文献 | 1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92. 2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5. 3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6. 4. Gospodarowicz D. 1974. Nature. 249:123-7. 5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700. 6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57. 9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29. |
| 研究领域 | |
| 研究领域 | CancerGrowth factorsFGF CardiovascularAngiogenesisGrowth FactorsFGFFGF NeuroscienceProcesses Signal TransductionGrowth Factors/HormonesFGF |
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