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Recombinant Human aFGF

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二抗、标签抗体、对照抗体;WB相关:ECL发光液、预染marker、预制胶;IHC相关:二抗试剂盒、组化笔;IP/CoIP试剂盒;细胞因子、信号通路调节剂;血清、BSA、蛋白酶K、CTB、TTX、CEE;凋亡试剂盒、呼吸爆发试剂盒;动植物提取物...

  • 产品型号:abs00875
  • 厂商性质:生产厂家
  • 更新时间:2022-11-16
  • 访  问  量:447

详细介绍

品牌自营品牌货号abs00875
规格10ug供货周期一周
概述
种属Human
形态Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
来源Escherichia coli.
氨基酸序列MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
内毒素水平Less than 1 EU/µg of Recombinant HumanaFGF as determined by LAL method.
活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/ml of heparin.
Accession #P05230
预期分子量Approximay 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
性能
溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度> 95 % by SDS-PAGE and HPLC analyses.
Gene IDs2246
参考文献

1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92.

2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5.

3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

4. Gospodarowicz D. 1974. Nature. 249:123-7.

5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.

6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

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