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Recombinant Human bFGF

简要描述:>>主要产品线:生化试剂(60万种)、抗体(10万种)、细胞因子、二抗、ECL发光液、蛋白marker、激动剂、抑制剂、凋亡试剂盒、BSA、动植物提取剂、蛋白酶K....

  • 产品型号:abs00877
  • 厂商性质:生产厂家
  • 更新时间:2022-11-16
  • 访  问  量:444

详细介绍

品牌自营品牌货号abs00877
规格10ug供货周期一周
概述
种属Human
形态Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
来源Escherichia coli.
氨基酸序列MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
内毒素水平Less than 1 EU/µg of Recombinant HumanbFGF as determined by LAL method.
活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
Accession #P09038
预期分子量Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
性能
溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度> 96 % by SDS-PAGE and HPLC analyses.
Gene IDs2247
参考文献
1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2. Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

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