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详细介绍
| 品牌 | absin | 纯度 | > 96 % |
|---|---|---|---|
| 货号 | abs00877 | 规格 | 10ug |
| 供货周期 | 现货 | 主要用途 | Lyophilized from a 0.2 μm filtered conce |
| 应用领域 | 化工,生物产业,农林牧渔,制药/生物制药,综合 |
Recombinant Human bFGF Protein
| 概述 | |
| 别名 | bFGF蛋白 |
| 种属 | Human |
| 来源 | Escherichia coli. |
| 形态 | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl. |
| 性能 | |
| 序列 | MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS |
| 标签 | No Tag |
| 纯度 | > 96 % by SDS-PAGE and HPLC analyses. |
| 内毒素水平 | Less than 1 EU/μg of Recombinant HumanbFGF as determined by LAL method. |
| 活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is ≤10 ng/ml. |
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 储存/保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 靶标 | |
| Accession | P09038 |
| Gene IDs | 2247 |
| 参考文献 | |
| 参考文献 | 1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6. 2. Gospodarowicz D. 1974. Nature. 249:123-7. 3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57. 6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29. 7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9. |
| 研究领域 | |
| 研究领域 | Developmental BiologyOrganogenesisExcretory system developmentKidney development NeuroscienceNeurology processNeurogenesis Stem CellsNeural Stem CellsExtracellular |
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