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Recombinant Bovine bFGF  10ug

Recombinant Bovine bFGF 10ug

型    号: abs01093
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abs01093Recombinant Bovine bFGF 10ug的详细资料:

概述
描述 
别名 
种属Bovine
形态Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
来源Escherichia coli.
氨基酸序列MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS
内毒素水平Less than 1 EU/µg of rBobFGF as determined by LAL method.
活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107IU/mg.
Accession #P03969
预期分子量Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
SDS-PAGE 
性能
溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度> 97 % by SDS-PAGE and HPLC analyses.
Gene IDs281161

参考文献

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2. Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.


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