Rabbit anti-β-Actin Polyclonal Antibody

简要描述：Rabbit anti-β-Actin Polyclonal Antibody，宿主Rabbit，特异性beta-Actin (Loading Control)，应用WB: 1:5000-50000, ICC: 1:100, FCM: 1ug/T, ELISA: 1:5000-20000

产品型号：abs119600
厂商性质：生产厂家
更新时间：2025-10-28
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药物阳性对照抗体磷酸化抗体二抗病毒抗体标签抗体内参抗体其它一抗

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详细介绍

品牌	absin	货号	abs119600
规格	50uL;100uL	供货周期	现货
主要用途	WB: 1:5000-50000, ICC: 1:100

概述
别名	β Actin抗体;Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin; beta; Beta actin; beta cytoskeletal actin; A X actin like protein; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN,betaActin
宿主	Rabbit
特异性	beta-Actin (Loading Control)
反应种属	Human, Mouse, Rat, Hamster
预测反应种属	Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat
应用	WB: 1:5000-50000, IHC-F: 1:200-1000, IHC-P: 1:200-1000, IF: 1:200-1000, ICC/IF: 1:100-500, FCM: 1ug/Test
分子量	Predicted molecular weight: 42kDa
免疫原	Synthetic MAP peptide derived from human beta-Actin(1-200/375)
性能
形式	Liquid
浓度	1mg/mL
纯化方法	affinity purified by Protein A
类型	Polyclonal Antibody
克隆号
同种型	IgG
储存/保存方法	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储溶液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
标记	Unlabeled
修饰	Unmodified
靶标
背景说明	Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.
组织特异性	Ubiquitously expressed in all eukaryotic cells.
翻译后修饰	ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
细胞定位	细胞浆
UniProt	P60709

研究领域
研究领域	Signal TransductionCytoskeleton / ECMCytoskeletonMicrofilamentsActin etcActin Tags & Cell MarkersSubcellular MarkersCytoskeletonActin

实验结果图

Sample:Embryo Cerebrum (Mouse) Lysate at 40 ug Du145 (Human) Lysate at 40 ug SW480 (Human) Cell Lysate at 40 ug U87MG (Human) Lysate at 40 ug U251 (Human) Lysate at 40 ug A673 (Human) Lysate at 40 ug Lovo (Human) Lysate at 40 ug 293FT (Human) Lysate at 40 ug JEG-3 (Human) Lysate at 40 ug RSC96 (Rat) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 40 ug HepG2 (Human) Lysate at 40 ug A431 (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody Dilution: 1ug/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1ug/T. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Rabbit anti-β-Actin Polyclonal Antibody

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