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Rabbit Anti-beta Actin Polyclonal Antibody(50ul)

Rabbit Anti-beta Actin Polyclonal Antibody(50ul)

型    号: abs119600
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abs119600Rabbit Anti-beta Actin Polyclonal Antibody(50ul)的详细资料:

概述
别名Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1.  
克隆类别Polyclonal
同种亚型IgG
反应种属 

Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep , Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
来源宿主Rabbit
应用

WB,ELISA,IHC-P,IHC-F,Flow-Cyt,IF
形态Lyophilized or Liquid
特异性beta-Actin (Loading Control)
使用方法WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 


not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.
性能
生产说明Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
背景Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].
保存方法Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
浓度1mg/1ml
研究领域肿瘤;细胞生物;信号转导;细胞骨架;
Gene IDsActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
分子量42kDa
功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

实验结果图

Sample: <br>A549 Cell (Human) Lysate at 30 ug<br>Primary: Lane1: Anti-beta-actin at 1/500 dilution<br>Lane2: Anti-beta-actin at 1/1000 dilution<br>Lane3: Anti-beta-actin at 1/2000 dilution<br>Lane4: Anti-beta-actin at 1/4000 dilution<br>Lane5: Anti-beta-actin at 1/5000 dilution<br>Lane6: Anti-beta-actin at 1/8000 dilution<br>Lane7: Anti-beta-actin at 1/10000 dilution<br>Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br>Predicted band size: 42 kD<br>Observed band size: 47 kD<br>

Sample: 
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-actin at 1/500 dilution
Lane2: Anti-beta-actin at 1/1000 dilution
Lane3: Anti-beta-actin at 1/2000 dilution
Lane4: Anti-beta-actin at 1/4000 dilution
Lane5: Anti-beta-actin at 1/5000 dilution
Lane6: Anti-beta-actin at 1/8000 dilution
Lane7: Anti-beta-actin at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD

Sample: <br>A549 Cell (Human) Lysate at 30 ug<br>Primary: Lane1: Anti-beta-actin at 1/500 dilution<br>Lane2: Anti-beta-actin at 1/1000 dilution<br>Lane3: Anti-beta-actin at 1/2000 dilution<br>Lane4: Anti-beta-actin at 1/4000 dilution<br>Lane5: Anti-beta-actin at 1/5000 dilution<br>Lane6: Anti-beta-actin at 1/8000 dilution<br>Lane7: Anti-beta-actin at 1/10000 dilution<br>Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br>Predicted band size: 42 kD<br>Observed band size: 47 kD<br>

Sample: 
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-actin at 1/500 dilution
Lane2: Anti-beta-actin at 1/1000 dilution
Lane3: Anti-beta-actin at 1/2000 dilution
Lane4: Anti-beta-actin at 1/4000 dilution
Lane5: Anti-beta-actin at 1/5000 dilution
Lane6: Anti-beta-actin at 1/8000 dilution
Lane7: Anti-beta-actin at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD

Sample: 
Lane1: 293T Cell Lysate at 25 ug
Lane2: A549 Cell Lysate at 25 ug
Lane3: A431 Cell Lysate at 25 ug
Primary: Anti- beta-Actin at 1/1000 and 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD

Sample: Lung lysate at 30ug;<br>Primary: Anti-beta-actin at 1:1000 dilution <br>Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution<br>Predicted band size : 42kD<br>Observed band size : 42kD<br>

Sample: Lung lysate at 30ug;
Primary: Anti-beta-actin at 1:1000 dilution 
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution
Predicted band size : 42kD
Observed band size : 42kD

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; <br>Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; <br>Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated secondary primary antibody 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining<br>

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; 
Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated secondary primary antibody 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Blank control: RSC96(blue). 
Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.


上一个 : abs132001Beta actin antibody(50ug)    下一个 :  abs137975beta-Actin Mouse Monoclonal Antibody(50ug)
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