概述 | |
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描述 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. |
别名 | ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1 |
克隆类别 | Polyclonal |
反应种属 | Zebrafish,Dog,Monkey,Human,Mouse,Rat,Pig |
来源宿主 | Rabbit |
应用 | WB 1:1000-1:10000 IHC 1:200 IF:200 |
特异性 | Beta actin antibody detects endogenous levels of total Beta actin |
性能 | |
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保存方法 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
浓度 | 1mg/ml |
分子量 | 43kDa |
功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
纯化方法 | Immunogen affinity purified |
亚细胞定位 | Cytoplasm,Cytoskeleton,Note:Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
序列 | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFMWISKQ EYDESGPSIVHRKCF |
实验结果图
Western blot analysis of Beta actin expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.