Rabbit anti-KRAS Polyclonal Antibody

简要描述：Rabbit anti-KRAS Polyclonal Antibody,WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.

产品型号：abs115469
厂商性质：生产厂家
更新时间：2025-11-05
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抗体

药物阳性对照抗体磷酸化抗体二抗病毒抗体标签抗体内参抗体其它一抗

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详细介绍

品牌	absin	货号	abs115469
规格	50uL	供货周期	现货
主要用途	WB:1:500-1:1000, IF/ICC:1:50-1:200	应用领域	化工,生物产业,农林牧渔,制药/生物制药,综合

Rabbit anti-KRAS Polyclonal Antibody

概述
别名	KRAS抗体;NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; KRAS,KRAS
宿主	Rabbit
反应种属	Human, Mouse, Rat
应用	WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.
分子量	Detects a band of approximately 21kDa (Predicted molecular weight22kDa)
免疫原	A synthetic peptide corresponding to a sequence within amino acids 30-189 of human KRAS (NP_203524.1).
阳性对照	A-549,NIH/3T3,Raji,HeLa,Mouse spleen,Mouse brain,Mouse lung,Rat brain
性能
形式	Liquid
纯化方法	Affinity purification
类型	Polyclonal Antibody
同种型	IgG
储存/保存方法	Store at -20℃. Avoid freeze / thaw cycles.
存储溶液	PBS with 0.05% proclin300,50% glycerol,pH7.3.
标记	Unlabeled
修饰	Unmodified
靶标
背景说明	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
细胞定位	Cell membrane,Cytoplasm,Cytoplasmic side,Lipid-anchor,cytosol
UniProt	P01116

实验结果图

Western blot analysis of various lysates using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

Western blot analysis of lysates from C6 cells, using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.