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品牌 | absin | 供货周期 | 现货 |
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应用领域 | 化工,生物产业,能源 |
概述 | |
别名 | |
宿主 | |
特异性 | |
反应种属 | |
预测反应种属 | |
应用 | |
免疫原 | |
性能 | |
浓度 | |
纯化方法 | |
类型 | |
同种型 | |
储存/保存方法 | |
存储溶液 | |
研究领域 | |
靶标 | |
背景说明 | |
细胞定位 | |
UniProt | |
实验结果图 | |
Sample: Brain (Mouse) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD | |
Sample: uterus (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 27 kD | |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. | |
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