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首页 > 产品中心 > 抗体 > 其它一抗 > abs126104Rabbit anti-IL-1β Polyclonal Antibody

Rabbit anti-IL-1β Polyclonal Antibody

简要描述:Rabbit anti-IL-1β Polyclonal Antibody,应用FCM: 2ug/T,分子量Predicted molecular weight: 17kD/32kDa,免疫原KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269

  • 产品型号:abs126104
  • 厂商性质:生产厂家
  • 更新时间:2025-10-28
  • 访  问  量:1764

详细介绍

品牌absin货号abs126104
规格50uL;100uL供货周期现货
主要用途FCM: 2ug/T
概述
别名
IL-1 β抗体;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta,IL1 beta
宿主
Rabbit
特异性
IL-1 Beta
反应种属
Human, Mouse, Rat
预测反应种属
Dog, Horse, Rabbit
应用
WB: 1:500-2000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, FCM: 2ug/Test
分子量
Predicted molecular weight: 17kD/32kDa
免疫原
KLH conjugated synthetic peptide derived from human IL-1 Beta(161-269/269)
Rabbit anti-IL-1β Polyclonal Antibody性能
形式
Liquid
浓度
1mg/mL
纯化方法
affinity purified by Protein A
类型
Polyclonal Antibody
克隆号
同种型
IgG
储存/保存方法
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储溶液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
标记
Unlabeled
修饰
Unmodified
靶标
背景说明
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
细胞定位
分泌型蛋白
UniProt
P01584
研究领域
研究领域
CardiovascularAtherosclerosisVascular InflammationInflammatory mediators
ImmunologyInnate ImmunityCytokinesInterleukins
MetabolismTypes of diseaseObesity
NeuroscienceProcesses
MicrobiologyOrganismVirusRNA VirusssRNA positive strand virusSARS Coronavirus
实验结果图
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Brain (Mouse) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgGHRP at 1/5000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-IL-1 Beta antibody Dilution: 2ug/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- IL-1 Beta at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD
Sample: Lane 1: Recombinant mouse IL-1b protein, C-His Primary: Anti-IL-1 Beta at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/32 kDa Observed band size: 25 kDa
Rabbit anti-IL-1β Polyclonal Antibody温馨提示:本产品仅作科研实验使用,不支持临床等研究

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