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首页 > 产品中心 > 抗体 > 二抗 > abs126104Rabbit Anti-IL-1 Beta Polyclonal Antibody

Rabbit Anti-IL-1 Beta Polyclonal Antibody

简要描述:Rabbit Anti-IL-1 Beta Polyclonal Antibody The protein encoded by this gene is a member of the interleukin 1 cytokine family.

  • 产品型号:abs126104
  • 厂商性质:生产厂家
  • 更新时间:2021-12-19
  • 访  问  量:74

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详细介绍

品牌自营品牌供货周期现货
应用领域化工,生物产业,能源
概述
别名
Rabbit Anti-IL-1 Beta Polyclonal Antibody IL-1 β抗体;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta.  
宿主
Rabbit
特异性
IL-1 Beta
反应种属
Mouse;Rat;
预测反应种属
Human;Dog;Horse;Rabbit;
应用
Rabbit Anti-IL-1 Beta Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
免疫原
KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269 
性能
浓度
1mg/ml
纯化方法
affinity purified by Protein A
类型
Polyclonal Antibody
同种型
IgG
储存/保存方法
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
存储溶液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
研究领域
细胞生物  免疫学  生长因子和激素  糖蛋白  
靶标
背景说明
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
细胞定位
分泌型蛋白 
UniProt
P01584
实验结果图
Sample:
Brain (Mouse) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 17/30 kD
Observed band size: 30 kD
Sample:
uterus (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/30 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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