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Donkey anti-Mouse IgG-AlexaFluor 488

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  • 产品型号:abs20014A
  • 厂商性质:生产厂家
  • 更新时间:2022-11-16
  • 访  问  量:446

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详细介绍

品牌自营品牌货号abs20014A
规格100ul供货周期一周
概述
描述

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

克隆类别Polyclonal
同种亚型Whole IgG
反应种属 Mouse
来源宿主Donkey
形态Liquid
特异性IgG (H+L)
使用方法
Histo-/Cyto-Chemistry:- 1:100-1:800 
Flow Cytometry:- 1:100-1:800 

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
性能
标识Alexa Fluor 488
保存方法Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid for 1 year.
存储溶液0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
0.05% Sodium Azide
浓度1mg/ml

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