Purified Mouse Monoclonal Antibody (Mab)
|别名||Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA|
|反应种属 ||H, R, M|
|形态||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|生产说明||This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein.|
|背景||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|保存方法||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Zhong R.,et al.Nucleic Acids Res. 11:7409-7425(1983).|
Marashi F.,et al.Biochem. Cell Biol. 64:277-289(1986).
Albig W.,et al.Genomics 10:940-948(1991).
Kardalinou E.,et al.J. Cell. Biochem. 52:375-383(1993).
Runge D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
Western blot analysis of lysates from Hela, MCF-7, C6 cell line (from left to right), using Histone Antibody (H3)(Cat. #abs100462). abs100462 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.