Mouse anti-β-Actin Monoclonal Antibody

β-Actin抗体;βActin

Mouse

beta-Actin antibody detects endogenous levels of total beta-Actin.

Human, Mouse, Rat, Monkey, Hamster

WB: 1:500-1:2000, ICC: 1:200-1:1000, FCM: 1:200-1:400, ELISA: 1:20000-1:40000

Predicted molecular weight: 43KD

Purified recombinant fragment of human beta-Actin expressed in E. Coli.

1mg/ml

Affinity-chromatography.

Monoclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

WB,IF/ICC,ELISA,FCM

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P60709