Rabbit anti-GAPDH Polyclonal Antibody

简要描述：Rabbit anti-GAPDH Polyclonal Antibody,WB: 1:10000-200000, IHC-P: 1:100-500, IHC-F: 1:100-500, IF: 1:100-500

产品型号：abs124037
厂商性质：生产厂家
更新时间：2025-10-29
访问量：1954

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详细介绍

品牌	absin	分子量	37kDa
货号	abs124037	规格	50uL
供货周期	两周	主要用途	WB: 1:10000-200000, IHC-P: 1:100-500,
应用领域	化工,生物产业,农林牧渔,制药/生物制药,综合

概述
别名	GAPDH抗体;GAPDH;Rabbit anti-GAPDH Polyclonal Antibody
宿主	Rabbit
特异性	GAPDH
反应种属	Human, Mouse, Rat, Rabbit, Porcine, Sheep, Goose, Chicken, Bovine, Pig, Rattus norvegicu, Duck, Broiler Chick, Cat, Zebrafish
应用	WB: 1:10000-200000, IHC-P: 1:100-500, IHC-F: 1:100-500, IF: 1:100-500
分子量	Predicted molecular weight: 37kDa
免疫原	GAPDH protein of rabbit
性能
形式	Liquid
浓度	1mg/mL
纯化方法	affinity purified by Protein A
类型	Polyclonal Antibody
同种型	IgG
储存/保存方法	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储溶液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
标记	Unlabeled
修饰	Unmodified
靶标
背景说明	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.
翻译后修饰	Acetylation
细胞定位	细胞骨架，细胞质，膜，细胞核
UniProt	P04406

研究领域
研究领域	Signal TransductionMetabolismEnergy Metabolism

实验结果图

Sample:
Hela Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Muscle (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (abs124037) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH(abs124037)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, and DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Rabbit anti-GAPDH Polyclonal Antibody温馨提示：本产品仅作科研实验使用，不支持临床等研究

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上一篇：abs137959Mouse anti-GAPDH Monoclonal 抗体
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Rabbit anti-GAPDH Polyclonal Antibody

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