Rabbit anti-GAPDH Polyclonal Antibody

GAPDH

Rabbit

Human, Mouse, Rat, Hamster

WB: 1:5000-50000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, ELISA: 1:5000-10000

Predicted molecular weight: 38kDa

Recombinant human GAPDH full length protein

Liquid

1mg/mL

affinity purified by Protein A

Polyclonal Antibody

IgG

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Unlabeled

Unmodified

oading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

细胞骨架,细胞质,膜,细胞核

P04406