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详细介绍
| 品牌 | absin | 供货周期 | 一周 |
|---|
| 概述 | |
|---|---|
| 描述 | DARPP-32 a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. |
| 别名 | DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B; |
| 克隆类别 | Polyclonal |
| 反应种属
| Human, Mouse, Rat |
| 来源宿主 | Rabbit |
| 应用
| WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500 |
| 特异性 | Phospho-DARPP-32 (Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Threonine 34 |
| 性能 | |
|---|---|
| 保存方法 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| 浓度 | 1mg/ml |
| 分子量 | 32kDa |
| 功能 | Inhibitor of protein-phosphatase 1. |
| 纯化方法 | Immunogen affinity purified |
| 亚细胞定位 | Cytoplasm, |
| 序列 | MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGE GHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREE DEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPPLDESERDGGSED QVEDPALSEPGEEPQRPSPSEPGT |
实验结果图
Western blot analysis of DARPP-32 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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