ECL化学发光检测试剂盒, 引用文献已达118篇

Absin ECL化学发光检测试剂盒产品详情

1. 基本属性

中文名称	ECL化学发光检测试剂盒
英文名字	ECL luminescence reagent
别名	ECL发光液；HRP化学发光底物
产品货号	abs920
产品规格	2*25ml，2*50ml，2*250ml
产品组份	A液；B液
保存建议	2-8℃避光保存6个月。
产品说明书

2. 产品描述

Western blot底物发光检测试剂可由标记于二抗上的辣根过氧化酶催化，产生化学发光反应，可以灵敏地检测出目的蛋白的存在。ECL底物化学发光检测试剂盒基于新一代增强型化学发光底物研制而成，并对成份做了优化。产品背景低，稳定性好。它由辣根过氧化物酶(HRP)催化发生化学反应，发出荧光，可对X光胶片曝光，也可直接进行luminometer检测或者荧光CCD扫描。

3. 操作步骤

1. 按常规Western blot操作，二抗孵育后，进行最后一次洗涤时，根据膜的大小，按每10 cm2 膜混合0.5ml溶液A和0.5ml溶液B混匀，配制成发光检测工作液。 
2. 用平头镊子将膜取出，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，置于洁净保鲜膜（某些市售保鲜膜包裹印迹膜时可能会淬灭荧光，应选择高质量保鲜膜）上。用吸管将配制的发光检测液转移到蛋白膜上，使其均匀覆盖，室温孵育2分钟。 
3. 用平头镊夹持蛋白膜，膜的下缘轻轻接触吸水纸，以去除膜上多余的液体。膜的蛋白面朝上，包裹于洁净保鲜膜内。轻轻赶出其间的气泡，固定在X片暗盒内。
4. 在暗室中取一张X片置于包裹的膜上，合上暗盒，曝光30秒至1分钟。立即显影定影，根据其曝光强度，缩短或延长下一张X片的曝光时间（对微弱信号，曝光时间可延长至数小时）。

4. 用法说明

1.ECL A液和B液在吸取过程中必须要更换枪头，A液和B液相互污染后会导致 A液或B液逐渐失效，影响后续的使用效果。
2.各溶液使用后，请盖紧瓶盖，以防失效。特别是B液，含有氧化剂，比较容易 被还原而失效。
3.ECL的荧光持续时间很长，但开始反应后的30分钟内荧光更强一些，随后荧光会逐渐减弱，因此请注意充分利用这荧光较强的30分钟进行压片。 
4.ECL A液和B液均对人体有害，操作时请注意适当防护。 为了您的安全和健康，请穿实验服并戴一次性手套操作。

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