beta-Tubulin Mouse Monoclonal Antibody

β-Tubulin抗体

Mouse

beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.

Human;Mouse;Rat;Sheep;Rabbit;Dog;Monkey;Hamster;Chicken

WB: 1:10000~1:500000, IHC: 1:200, IF/ICC: 1:200, ELISA(peptide) 1:20000-1:40000

Full-length beta-tubulin protein of human.

1mg/ml

Affinity-chromatography.

Monoclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

beta-Tubulin Mouse Monoclonal Antibody Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Cytoskeleton;Extracellular region or secreted;Nucleus;

Q13509