| 概述 |
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| 描述 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. |
| 别名 | Beta actin antibody β-Actin抗体 |
| 宿主 | Rabbit |
| 特异性 | Beta actin antibody detects endogenous levels of total Beta actin. |
| 反应种属 | Human;Mouse;Rat;Pig;Zebrafish;Dog;Monkey;Fish |
| 预测反应种属 | Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse; |
| 应用 | WB 1:3000-1:20000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 |
| 免疫原 | A synthesized peptide derived from human Beta actin. |
| 性能 |
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| 浓度 | 1mg/ml |
| 纯化方法 | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| 类型 | Polyclonal Antibody |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| 存储溶液 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
| 靶标 |
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| 背景说明 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 翻译后修饰 | ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
| 细胞定位 | Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane; |
| UniProt | P60709 |
| 实验结果图 |
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| Western blot analysis of Beta actin expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. |
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