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| 品牌 | absin | 供货周期 | 一周 |
|---|
| 概述 | |
|---|---|
| 描述 | beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues. |
| 别名 | ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1 |
| 克隆类别 | Monoclonal |
| 反应种属
| Human; Monkey; Mouse; Rat; hamster; Dog; Spodoptera frugiperda; chicken，rabbit.Pg |
| 来源宿主 | Mouse |
| 应用
| WB: 1:3000~1:10000, IHC: 1:200 , IF/ICC: 1:200 |
| 特异性 | beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein. |
| 性能 | |
|---|---|
| 保存方法 | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| 浓度 | 1mg/ml |
| 分子量 | 43KD |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 纯化方法 | Affinity-chromatography |
| 亚细胞定位 | Cytoplasm,Cytoskeleton,Note:Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| 序列 | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFMWISKQ EYDESGPSIVHRKCF |
实验结果图
Western blot analysis of Hela (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5)with beta-Actin mouse mAb.
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