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beta-Actin Mouse Monoclonal Antibody(50ug)

beta-Actin Mouse Monoclonal Antibody(50ug)

型    号: abs137975
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abs137975beta-Actin Mouse Monoclonal Antibody(50ug)的详细资料:

概述
描述beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
别名ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1
克隆类别Monoclonal
反应种属 

Human; Monkey; Mouse; Rat; hamster; Dog; Spodoptera frugiperda; chicken,rabbit.Pg
来源宿主Mouse
应用

WB: 1:3000~1:10000, IHC: 1:200 , IF/ICC: 1:200
特异性beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.
性能
保存方法Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
浓度1mg/ml
分子量43KD
功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
纯化方法Affinity-chromatography
亚细胞定位Cytoplasm,Cytoskeleton,Note:Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
序列MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFMWISKQ EYDESGPSIVHRKCF

实验结果图

Western blot analysis of Hela (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5)with beta-Actin mouse mAb.

Western blot analysis of Hela (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5)with beta-Actin mouse mAb.


上一个 : abs119600Rabbit Anti-beta Actin Polyclonal Antibody(50ul)    下一个 :  abs112431Beta-Actin Antibody(50ul)
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