Recombinant human PD1 protein aa1-160 was produced in mammalian cells with C-terminal DYK tag. The theoretical molecular weight of recombinant PD1 is 16.6 kDa. Purified PD1 protein is near the position of 34 kDa on SDS-PAGE gel under reduced condition.
PD1 protein is purified by proprietary chromatographic techniques.
Entrez Gene summary for PD1:
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis.
UniProtKB/Swiss-Prot: Q15116 (PDCD1_HUMAN)
Function: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
形态 Protein is supplied in 50 mM Tris, 150 mM NaCl, 10% Glycerol, 150 ug/ml Flag peptide, pH 7.5.
Accession # NP_005009.2
保存方法 Clear solution, frozen.Store at -70°C. At first use, aliquot and store at -70°C to avoid multiple freeze-thaws.
序列 PD1-DYK (N-terminal 20 aa is predicted to be signal peptide): MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPDYKDDDDK