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品牌 | absin | 货号 | abs00937 |
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规格 | 2ug | 供货周期 | 一周 |
概述 | |
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种属 | Human |
形态 | Lyophilized from a 0.2 µm filtered concentrated solution containing 10 mM sodium citrate pH 3.5. |
来源 | Escherichia coli. |
氨基酸序列 | MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPSAISM LYLDENEKVV LKNYQDMVVE GCGCR |
内毒素水平 | Less than 1 EU/µg of Recombinant HumanBMP-2 as determined by LAL method. |
活性 | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 103 IU/mg. |
Accession # | P12643 |
预期分子量 | Approximay 26.0 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains. |
性能 | |
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溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media. |
保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
纯度 | > 95 % by SDS-PAGE and HPLC analyses. |
Gene IDs | 650 |
参考文献 | |
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1. Chung YI, Ahn KM, Jeon SH, et al. 2007. J Control Release, 121: 91-9.<br/>2. Yamaji K, Kawanami M, Matsumoto A, et al. 2007. Dent Mater J, 26: 481-6.<br/>3. Allen RT, Lee YP, Stimson E, et al. 2007. Spine (Phila Pa 1976), 32: 2996-3006.<br/>4. Shen B, Wei A, Tao H, et al. 2009. Tissue Eng Part A, 15: 1311-20.<br/>5. Seib FP, Lanfer B, Bornhauser M, et al. 2010. J Tissue Eng Regen Med, 4: 324-7.<br/>6. Itoh T, Ando M, Tsukamasa Y, et al. 2012. FEBS Lett, 586: 1693-701. |
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